PMID:11741997
| Citation |
Xia, B, Ke, H, Jiang, W and Inouye, M (2002) The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature. J. Biol. Chem. 277:6005-11 |
|---|---|
| Abstract |
The 5'-end region of cspA mRNA contains a Cold Box sequence conserved among several cold-shock mRNAs. This region forms a stable stem-loop structure followed by an AU-rich sequence. Here we show that the Cold Box region is essential for the normal scale of cspA mRNA induction after cold shock because a deletion of the stem-loop significantly destabilizes the mRNA and reduces the cold shock-induced cspA mRNA amount by approximately 50%. The AU-rich track, however, slightly destabilizes the mRNA. The integrity of the stem is essential for the stabilizing function, whereas that of the loop sequence is less important. Overexpression of a mutant cspA mRNA devoid of both the AUG initiation codon and the coding sequence results in a severe growth inhibition at low temperature along with a derepression of the chromosomal cspA expression. Furthermore, the overexpressed RNA is stably associated with the 30 S and 70 S ribosomes. Our results demonstrate that the AUG initiation codon and the coding region containing the downstream box are not required for cspA mRNA to bind ribosomes and that the 5'-untranslated region by itself has a remarkable affinity to ribosomes at low temperature. |
| Links |
PubMed Online version:10.1074/jbc.M109700200 |
| Keywords |
5' Untranslated Regions/genetics; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cold Temperature; Conserved Sequence; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Kinetics; Molecular Sequence Data; Nucleic Acid Conformation; Open Reading Frames; RNA, Messenger/chemistry; RNA, Messenger/genetics; Sequence Deletion |
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