PMID:11738611
| Citation |
Schönleber, RO, Bendig, J, Hagen, V and Giese, B (2002) Rapid photolytic release of cytidine 5'-diphosphate from a coumarin derivative: a new tool for the investigation of ribonucleotide reductases. Bioorg. Med. Chem. 10:97-101 |
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| Abstract |
In order to study the long-range radical transfer in the Escherichia coli ribonucleotide reductase (RNR), caged cytidine 5'-diphosphate (CDP) 1 was synthesized, which contains the photolabile (7-diethylaminocoumarin-4-yl)methyl moiety. The caged CDP 1 triggers the release of CDP when irradiated at wavelengths between 365 and 436 nm. The rate constant of the formation of alcohol 2 and cytidine 5'-diphosphate 3 is 2x10(8) s(-1) and the quantum efficiency for the disappearance of caged CDP 1 is 2.9%. |
| Links | |
| Keywords |
Coumarins/chemistry; Cytidine Diphosphate/chemistry; Hydrolysis; Mass Spectrometry/methods; Ribonucleotide Reductases/chemistry |
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