PMID:11726509
| Citation |
Voulhoux, R, Ball, G, Ize, B, Vasil, ML, Lazdunski, A, Wu, LF and Filloux, A (2001) Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. EMBO J. 20:6735-41 |
|---|---|
| Abstract |
The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system, which involves machinery called the secreton. This step is considered to be an extension of the general export pathway, i.e. the export of proteins across the inner membrane by the Sec machinery. Here, we demonstrate that two substrates for the Pseudomonas aeruginosa secreton, both phospholipases, use the twin-arginine translocation (Tat) system, instead of the Sec system, for the first step of translocation across the inner membrane. These results challenge the previous vision of the GSP and suggest for the first time a mosaic model in which both the Sec and the Tat systems feed substrates into the secreton. Moreover, since P.aeruginosa phospholipases are secreted virulence factors, the Tat system appears to be a novel determinant of bacterial virulence. |
| Links |
PubMed PMC125745 Online version:10.1093/emboj/20.23.6735 |
| Keywords |
Amino Acid Sequence; Arginine/chemistry; Arginine/metabolism; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Immunoblotting; Mass Spectrometry; Models, Biological; Molecular Sequence Data; Multigene Family; Mutation; Phospholipases/metabolism; Plasmids/metabolism; Protein Transport; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/metabolism; Pseudomonas aeruginosa/pathogenicity; Type C Phospholipases/metabolism |
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