PMID:11703658
| Citation |
Journet, L, Bouveret, E, Rigal, A, Lloubes, R, Lazdunski, C and Bénédetti, H (2001) Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm. Mol. Microbiol. 42:331-44 |
|---|---|
| Abstract |
Several proteins of the Tol/Pal system are required for group A colicin import into Escherichia coli. Colicin A interacts with TolA and TolB via distinct regions of its N-terminal domain. Both interactions are required for colicin translocation. Using in vivo and in vitro approaches, we show in this study that colicin A also interacts with a third component of the Tol/Pal system required for colicin import, TolR. This interaction is specific to colicins dependent on TolR for their translocation, strongly suggesting a direct involvement of the interaction in the colicin translocation step. TolR is anchored to the inner membrane by a single transmembrane segment and protrudes into the periplasm. The interaction involves part of the periplasmic domain of TolR and a small region of the colicin A N-terminal domain. This region and the other regions responsible for the interaction with TolA and TolB have been mapped precisely within the colicin A N-terminal domain and appear to be arranged linearly in the colicin sequence. Multiple contacts with periplasmic-exposed Tol proteins are therefore a general principle required for group A colicin translocation. |
| Links | |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane/metabolism; Colicins/chemistry; Colicins/genetics; Colicins/metabolism; Cross-Linking Reagents/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Formaldehyde/metabolism; Membrane Proteins/metabolism; Periplasm/metabolism; Periplasmic Proteins; Plasmids/genetics; Precipitin Tests; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Protein Transport |
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