PMID:11702079
| Citation |
Hoffer, SM, van Uden, N and Tommassen, J (2001) Expression of the pho regulon interferes with induction of the uhpT gene in Escherichia coli K-12. Arch. Microbiol. 176:370-6 |
|---|---|
| Abstract |
The uptake of hexose 6-phosphates in Escherichia coli is mediated by the transporter UhpT, the synthesis of which is induced by the presence of glucose 6-phosphate (glucose 6-P) in the medium. Since this protein functions as an anion exchanger, it is generally assumed to be geared for the use of sugar phosphates as a carbon source. However, the question was unresolved whether this transporter can also provide the cells with glucose 6-P as a phosphate source. It is demonstrated in this work that UhpT-mediated glucose 6-P uptake does not allow the cells to grow on glucose 6-P as phosphate source. Hence, the expression of UhpT under phosphate limitation would not be particularly advantageous and some form of interaction between the uhp system and the Pi-limitation-inducible pho regulon, the products of which are involved in phosphate acquisition, may be anticipated. Indeed, the use of an uhpT-lacZ fusion revealed that much higher concentrations of the inducer glucose 6-P were required to elevate uhpT transcription when the pho regulon was expressed. This interference was the result of degradation of glucose 6-P by one of the products of the pho regulon, the periplasmic enzyme alkaline phosphatase. The specific form of interaction between the Pho and Uhp systems is designated inducer degradation. |
| Links |
PubMed Online version:10.1007/s002030100339 |
| Keywords |
Alkaline Phosphatase/metabolism; Down-Regulation/physiology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Glucose-6-Phosphate/metabolism; Monosaccharide Transport Proteins/genetics; Monosaccharide Transport Proteins/metabolism; Mutation; Phosphates/metabolism; Regulon/physiology |
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