PMID:11701448
| Citation |
Thommesen, L, Hofsli, E, Paulssen, RH, Anthonsen, MW and Laegreid, A (2001) Molecular mechanisms involved in gastrin-mediated regulation of cAMP-responsive promoter elements. Am. J. Physiol. Endocrinol. Metab. 281:E1316-25 |
|---|---|
| Abstract |
In the present study, we explore the role of cAMP-responsive (CRE) promoter elements in gastrin-mediated gene activation. By using the minimal CRE promoter reporter plasmid, pCRELuc, we show that gastrin can activate CRE. This activation is blocked by H-89 and GF 109203x, which inhibit protein kinases A and C, respectively. Moreover, Ca(2+)-activated pathways seem to be involved, because the calmodulin inhibitor W-7 reduced gastrin-mediated activation of pCRELuc. Deletion of CRE from the c-fos promoter rendered this promoter completely unresponsive to gastrin, indicating that CRE plays a central role in c-fos transactivation. Interestingly, gastrin-induced expression of the inducible cAMP early repressor (ICER), a gene that is known to be regulated by CRE promoter elements, was not reduced by H-89, W-7, or GF 109203x. Furthermore, bandshift analyses indicated that the region of the ICER promoter containing the CRE-like elements CARE 3-4 binds transcription factors that are not members of the CRE-binding protein-CRE modulator protein-activating transcription factor, or CREB/CREM/ATF-1, family. Our results underline the significance of the CRE promoter element in gastrin-mediated gene regulation and indicate that a variety of signaling mechanisms are involved, depending on the CRE promoter context. |
| Links | |
| Keywords |
Animals; Cell Division/drug effects; Cell Division/physiology; Cells, Cultured; Cyclic AMP/metabolism; Cyclic AMP-Dependent Protein Kinases/metabolism; Gastrins/physiology; Gene Expression Regulation/physiology; Genes, Reporter/genetics; Genes, fos/genetics; Luciferases/metabolism; Plasmids/genetics; Promoter Regions, Genetic/genetics; Protein Kinase C/metabolism; Rats; Response Elements/genetics; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction/physiology; Tetradecanoylphorbol Acetate/pharmacology; Transcription Factors/genetics; Transcriptional Activation; Transfection |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.