PMID:11701035
| Citation |
Hecht, S, Wungsintaweekul, J, Rohdich, F, Kis, K, Radykewicz, T, Schuhr, CA, Eisenreich, W, Richter, G and Bacher, A (2001) Biosynthesis of terpenoids: efficient multistep biotransformation procedures affording isotope-labeled 2C-methyl-D-erythritol 4-phosphate using recombinant 2C-methyl-D-erythritol 4-phosphate synthase. J. Org. Chem. 66:7770-5 |
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| Abstract |
This paper describes the recombinant expression of the ispC gene of Escherichia coli specifying 2C-methyl-D-erythritol 4-phosphate synthase in a modified form that can be purified efficiently by metal-chelating chromatography. The enzyme was used for the preparation of isotope-labeled 2C-methyl-D-erythritol 4-phosphate employing isotope-labeled glucose and pyruvate as starting materials. The simple one-pot methods described afford numerous isotopomers of 2C-methyl-D-erythritol 4-phosphate carrying (3)H, (13)C, or (14)C from commercially available precursors. The overall yield based on the respective isotope-labeled starting material is approximately 50%. |
| Links | |
| Keywords |
Aldose-Ketose Isomerases/chemistry; Aldose-Ketose Isomerases/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Biotransformation; Carbon Radioisotopes/metabolism; Erythritol/analogs & derivatives; Erythritol/chemistry; Erythritol/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Isotope Labeling/methods; Multienzyme Complexes/chemistry; Multienzyme Complexes/metabolism; Oxidoreductases/chemistry; Oxidoreductases/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Sugar Phosphates/chemistry; Sugar Phosphates/metabolism; Terpenes/chemistry; Terpenes/metabolism; Tritium/metabolism |
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