PMID:11696367
| Citation |
Maeshima, H, Okuno, E, Aimi, T, Morinaga, T and Itoh, T (2001) An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA. FEBS Lett. 507:336-40 |
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| Abstract |
The gene encoding the 54 kDa protein of signal recognition particle (SRP54) in the hyperthermophilic archaeon Pyrococcus furiosus has been cloned and sequenced. Recombinant P. furiosus SRP54 (pf-SRP54) and the N-terminal G-domain and C-terminal M-domain (pf-SRP54M) of pf-SRP54 with an amino-terminal addition of six histidine residues were expressed in Escherichia coli and subjected to binding experiments for SRP RNA, non-conserved 213-nucleotide RNA (helices 1, 2, 3, 4 and 5) and conserved 107-nucleotide RNA (helices 6 and 8) from SRP RNA. The RNA binding properties of the purified protein were determined by filter binding assays. The histidine-tagged pf-SRP54M bound specifically to the conserved 107-nucleotide RNA in the absence of pf-SRP19, unlike the eukaryotic homologue, with an apparent binding constant (K) of 18 nM. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Archaeal Proteins/genetics; Archaeal Proteins/metabolism; Base Sequence; Binding Sites; Cloning, Molecular; Conserved Sequence; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Humans; Mammals; Methionine; Molecular Sequence Data; Pyrococcus furiosus/genetics; RNA/metabolism; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Signal Recognition Particle/genetics; Signal Recognition Particle/metabolism |
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