PMID:11682179
| Citation |
Zakharyan, E and Trchounian, A (2001) K+ influx by Kup in Escherichia coli is accompanied by a decrease in H+ efflux. FEMS Microbiol. Lett. 204:61-4 |
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| Abstract |
Escherichia coli accumulates K+ by means of multiple uptake systems of which Kup is the major transport system at acidic pH. In cells grown under fermentative conditions at pH 5.5, K+ influx by a wild-type strain upon hyper-osmotic stress at pH 5.5 was accompanied by a marked decrease in H+ efflux, with a 1:1 ratio of K+ to H+ fluxes. This was observed with cells treated with N,N'-dicyclohexylcarbodiimide. Similar results with a mutant defective in Kdp and TrkA but with a functional Kup system but not in a mutant defective in Kdp and Kup but having an active TrkA system suggest that Kup operates as a H+ -K+ -symporter. |
| Links | |
| Keywords |
Adenosine Triphosphatases/metabolism; Carrier Proteins/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Hydrogen/metabolism; Hydrogen-Ion Concentration; Ion Transport; Membrane Proteins/metabolism; Osmotic Pressure; Potassium/metabolism; Proton-Motive Force |
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