PMID:11676470

Citation	Kamiya, H, Murata-Kamiya, N, Iida, E and Harashima, H (2001) Hydrolysis of oxidized nucleotides by the Escherichia coli Orf135 protein. Biochem. Biophys. Res. Commun. 288:499-502
Abstract	To examine the possibility that the Orf135 protein of Escherichia coli functions as a hydrolyzing enzyme for a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate), we purified the recombinant Orf135 protein and incubated it with oxidized deoxynucleotides. Of the nucleotides tested, 2-hydroxydeoxyadenosine 5'-triphosphate, and somewhat less efficiently, 8-hydroxydeoxyguanosine 5'-triphosphate, were hydrolyzed by this protein. These damaged deoxynucleotides elicit transversion mutations in E. coli (Inoue, M., Kamiya, H., Fujikawa, K., Ootsuyama, Y., Murata-Kamiya, N., Osaki, T., Yasumoto, K., Kasai, H. (1998) J. Biol. Chem. 273, 11069-11074). These results suggest that this protein may be involved in the prevention of mutations induced by these oxidized deoxynucleotides.
Links	PubMed Online version:10.1006/bbrc.2001.5781
Keywords	DNA Damage; Deoxyadenine Nucleotides/chemistry; Deoxyadenine Nucleotides/pharmacology; Deoxyguanine Nucleotides/chemistry; Deoxyguanine Nucleotides/pharmacology; Escherichia coli/enzymology; Hydrolysis; Nucleotides/metabolism; Oxidation-Reduction; Pyrophosphatases/antagonists & inhibitors; Pyrophosphatases/isolation & purification; Pyrophosphatases/metabolism
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