PMID:11567013
| Citation |
Cabrera, JE and Jin, DJ (2001) Growth phase and growth rate regulation of the rapA gene, encoding the RNA polymerase-associated protein RapA in Escherichia coli. J. Bacteriol. 183:6126-34 |
|---|---|
| Abstract |
The Escherichia coli rapA gene encodes the RNA polymerase (RNAP)-associated protein RapA, which is a bacterial member of the SWI/SNF helicase-like protein family. We have studied the rapA promoter and its regulation in vivo and determined the interaction between RNAP and the promoter in vitro. We have found that the expression of rapA is growth phase dependent, peaking at the early log phase. The growth phase control of rapA is determined at least by one particular feature of the promoter: it uses CTP as the transcription-initiating nucleotide instead of a purine, which is used for most E. coli promoters. We also found that the rapA promoter is subject to growth rate regulation in vivo and that it forms intrinsic unstable initiation complexes with RNAP in vitro. Furthermore, we have shown that a GC-rich or discriminator sequence between the -10 and +1 positions of the rapA promoter is responsible for its growth rate control and the instability of its initiation complexes with RNAP. |
| Links |
PubMed PMC99692 Online version:10.1128/JB.183.20.6126-6134.2001 |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Codon, Initiator; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Transcription, Genetic |
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