PMID:11557017
| Citation |
Maurer, JA and Dougherty, DA (2001) A high-throughput screen for MscL channel activity and mutational phenotyping. Biochim. Biophys. Acta 1514:165-9 |
|---|---|
| Abstract |
A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead BacLight bacterial viability assay to monitor MscL channel activity as a function of bacterial survival from osmotic downshock. |
| Links | |
| Keywords |
Cell Survival; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Fluorescence; Ion Channels/analysis; Ion Channels/genetics; Mutation; Osmotic Pressure; Phenotype; Propidium |
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