PMID:11532151
| Citation |
Langdon, RC, Burr, T, Pagan-Westphal, S and Hochschild, A (2001) A chimeric activator of transcription that uses two DNA-binding domains to make simultaneous contact with pairs of recognition sites. Mol. Microbiol. 41:885-96 |
|---|---|
| Abstract |
Many well-known transcriptional regulatory proteins are composed of at least two independently folding domains and, typically, only one of these is a DNA-binding domain. However, some transcriptional regulators have been described that have more than one DNA-binding domain. Regulators with a single DNA-binding domain often bind co-operatively to the DNA in homotypic or heterotypic combinations, and two or more DNA-binding domains of a single regulatory protein can also bind co-operatively to suitably positioned recognition sequences. Here, we examine the behaviour of a chimeric activator of transcription with two different DNA-binding domains, that of the bacteriophage lambda cI protein and that of the Escherichia coli cyclic AMP receptor protein. We show that these two DNA-binding moieties, when present in the same molecule, can bind co-operatively to a pair of cognate recognition sites located upstream of a test promoter, thereby permitting the chimera to function as a particularly strong activator of transcription from this promoter. Our results show how such a bivalent DNA-binding protein can be used to regulate transcription differentially from promoters that bear either one or both recognition sites. |
| Links | |
| Keywords |
Bacteriophage lambda; Binding Sites; Cyclic AMP Receptor Protein/chemistry; Cyclic AMP Receptor Protein/genetics; Cyclic AMP Receptor Protein/metabolism; DNA Footprinting; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; DNA-Directed RNA Polymerases/metabolism; Deoxyribonuclease I/metabolism; Electrophoretic Mobility Shift Assay; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Reporter; Models, Genetic; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Response Elements/genetics; Trans-Activators/chemistry; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.