PMID:11532026
| Citation |
Izutsu, K, Wada, A and Wada, C (2001) Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp. Genes Cells 6:665-76 |
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| Abstract |
During the transition from the logarithmic to the stationary phase, 70S ribosomes are dimerized into the 100S form, which has no translational activity. Ribosome Modulation Factor (RMF) is induced during the stationary phase and binds to the 50S ribosomal subunit, which directs the dimerization of 70S ribosomes. Unlike many other genes induced in the stationary phase, rmf transcription is independent of the sigma S. To identify the factors that regulate the growth phase-dependent induction of rmf, mutant strains deficient in global regulators were examined for lacZ expression directed by the rmf promoter. |
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| Keywords |
Cell Division; Cloning, Molecular; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial; Lac Operon; Mutation; Operon; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; Ribosomal Proteins/biosynthesis; Ribosomal Proteins/genetics; Ribosomes/metabolism; Transcription, Genetic |
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