PMID:11524953

Citation	Maĭvali, U, Saarma, U and Remme, Ia [Mutations in the Escherichia coli 23S rRNA increase the rate of peptidyl-tRNA dissociation from the ribosome]. Mol. Biol. (Mosk.) 35:666-71
Abstract	We have studied in vivo the phenotypes of 23S rRNA mutations G2582A, G2582U, G2583C, and U2584C, which are located at the A site of Escherichia coli 50S ribosomal subunit. All mutant rRNAs incorporated into 50S ribosomal subunits. Upon sucrose gradient fraction of cell lysates, 23S rRNAs mutated at G2582 to A and G2583 to C accumulated in the 50S and 70S fractions and were under-represented in the polysome fraction. Induction of 23S rRNAs mutated at G2582 and G2583 lead to a drastic reduction in cell growth. In addition, mutations G2582A and G2583C reduced to one-third the total protein synthesis but not the RNA synthesis. Finally, we show that 23S rRNA mutations G2582A, G2582U, and G2583C cause a significant increase in peptidyl-tRNA drop-off from ribosomes, thereby reducing translational processivity. The results clearly show that tRNA-23S rRNA interaction has an essential role in maintaining the processivity of translation.
Links	PubMed
Keywords	Escherichia coli/genetics; Escherichia coli/metabolism; Mutation; Protein Biosynthesis; RNA, Ribosomal, 23S/genetics; RNA, Ribosomal, 23S/metabolism; RNA, Transfer, Amino Acyl/genetics; RNA, Transfer, Amino Acyl/metabolism; Ribosomes/genetics; Ribosomes/metabolism
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