PMID:11517307
| Citation |
Ranquet, C, Geiselmann, J and Toussaint, A (2001) The tRNA function of SsrA contributes to controlling repression of bacteriophage Mu prophage. Proc. Natl. Acad. Sci. U.S.A. 98:10220-5 |
|---|---|
| Abstract |
The small regulatory RNA SsrA has both tRNA and mRNA activities. It charges alanine and interacts with stalled ribosomes, allowing for translation to resume on the SsrA mRNA moiety. Hence, unfinished peptides carry a short amino acid tag, which serves as a signal for degradation by energy-dependent proteases. In SsrA-defective Escherichia coli strains, thermoinducible mutants of the transposable bacteriophage Mu (Mucts) are no longer induced at high temperature. Here we show that truncated forms of the key regulator of Mu lysogeny, the repressor Repc, accumulate in the absence of SsrA. These forms resemble C-terminally truncated dominant Mu repressor mutants previously isolated from Mucts, which are no longer thermoinducible and bind operator DNA with a high affinity even at high temperature. Using various ssrA alleles, we demonstrate the importance of SsrA charging on the ribosome for controlling Mu prophage repression. Our results thus substantiate the previous observation that trans-translation is not the only function of the SsrA. The alternative function of SsrA appears to influence the stability of Mu lysogens by controlling the translation of the C-terminal domain of the repressor protein, which modulates the affinity of the protein for DNA and its susceptibility to proteolytic degradation. |
| Links |
PubMed PMC56942 Online version:10.1073/pnas.171620598 |
| Keywords |
Alanine/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage mu/genetics; Base Sequence; Chromosome Mapping; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Genes, Bacterial; Genome, Viral; Molecular Sequence Data; Mutation; Phenotype; Plasmids/genetics; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.