PMID:11506918
| Citation |
Laurinavichene, TV and Tsygankov, AA (2001) H2 consumption by Escherichia coli coupled via hydrogenase 1 or hydrogenase 2 to different terminal electron acceptors. FEMS Microbiol. Lett. 202:121-4 |
|---|---|
| Abstract |
Hydrogen uptake in the presence of various terminal electron acceptors was examined in Escherichia coli mutants synthesizing either hydrogenase 1 or hydrogenase 2. Both hydrogenases mediated nitrate-dependent H2 consumption but neither of them was coupled with nitrite. Unlike hydrogenase 2, hydrogenase 1 demonstrated poor activity with electron acceptors of low midpoint redox potential. Oxygen-linked H2 uptake via hydrogenase 1 was observed over a wide range of air concentrations. Hydrogenase 2 catalyzed this reaction only at low air concentrations. Thus, hydrogenase 1 works in cells at higher redox potential, being more tolerant to oxygen than hydrogenase 2. |
| Links | |
| Keywords |
Electron Transport; Enzyme Activation; Escherichia coli/enzymology; Escherichia coli/metabolism; Hydrogen/metabolism; Hydrogenase/isolation & purification; Hydrogenase/metabolism; Kinetics; Oxidation-Reduction; Oxidoreductases/isolation & purification; Oxidoreductases/metabolism; Oxygen/metabolism |
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