PMID:11478944

Citation	Lamla, T and Erdmann, VA (2001) In vitro selection of other proteins than antibodies by means of ribosome display. FEBS Lett. 502:35-40
Abstract	The in vitro selection and simultaneous evolution of proteins is feasible by means of ribosome display. Here, we describe the use of a protein bearing a binding property without being an antibody for affinity enrichment of the ternary complex, consisting of a protein, a ribosome and a encoding mRNA. The binding property was a simple affinity tag, namely Strep-tag II and His-tag. We could demonstrate that the selection of a specific mRNA encoding a shortened bovine heart fatty acid binding protein with a N-terminal His-tag was possible. After nine cycles of transcription, translation, affinity selection and reverse transcription PCR the protein with the His-tag could be enriched 10(8)-fold.
Links	PubMed
Keywords	Animals; Antibodies/metabolism; Carrier Proteins/biosynthesis; Carrier Proteins/genetics; Cattle; Chromatography, Affinity/methods; Fatty Acid-Binding Proteins; Neoplasm Proteins; Polymerase Chain Reaction; Puromycin/metabolism; RNA, Messenger/metabolism; Ribosomes/metabolism
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