PMID:11466284
| Citation |
Patzer, SI and Hantke, K (2001) Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding an NRAMP-like Mn(2+) transporter in Escherichia coli. J. Bacteriol. 183:4806-13 |
|---|---|
| Abstract |
The uptake of Mn(2+), a cofactor for several enzymes in Escherichia coli, is mediated by MntH, a proton-dependent metal transporter, which also recognizes Fe(2+) with lower affinity. MntH belongs to the NRAMP family of eukaryotic Fe(2+) and Mn(2+) transporters. In E. coli strains with chromosomal mntH-lacZ fusions, mntH was partially repressed by both Mn(2+) and Fe(2+). Inactivation of fur resulted in the loss of Fe(2+)-dependent repression of mntH transcription, demonstrating that Fe(2+) repression depends on the global iron regulator Fur. However, these fur mutants still showed Mn(2+)-dependent repression of mntH. The Mn(2+)-responsive transcriptional regulator of mntH was identified as the gene product of o155 (renamed MntR). mntR mutants were impaired in Mn(2+) but not Fe(2+) repression of mntH transcription. Binding of purified MntR to the mntH operator was manganese dependent. The binding region was localized by DNase I footprinting analysis and covers a nearly perfect palindrome. The Fur binding site, localized within 22 nucleotides of the mntH operator by in vivo operator titration assays, resembles the Fur-box consensus sequence. |
| Links |
PubMed PMC99535 Online version:10.1128/JB.183.16.4806-4813.2001 |
| Keywords |
Amino Acid Substitution; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cation Transport Proteins; Chromosome Mapping; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Iron/metabolism; Manganese/metabolism; Metalloproteins/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Recombinant Fusion Proteins/metabolism; Recombinant Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sequence Alignment; Sequence Homology, Nucleic Acid |
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