PMID:11447256
| Citation |
Becherel, OJ and Fuchs, RP (2001) Mechanism of DNA polymerase II-mediated frameshift mutagenesis. Proc. Natl. Acad. Sci. U.S.A. 98:8566-71 |
|---|---|
| Abstract |
Escherichia coli possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single N-2-acetylaminofluorene adduct within the NarI mutation hot spot. Indeed, error-free bypass requires Pol V (umuDC), whereas mutagenic (-2 frameshift) bypass depends on Pol II (polB). In this paper, we show that purified DNA Pol II is able in vitro to generate the -2 frameshift bypass product observed in vivo at the NarI sites. Although the Delta polB strain is completely defective in this mutation pathway, introduction of the polB gene on a low copy number plasmid restores the -2 frameshift pathway. In fact, modification of the relative copy number of polB versus umuDC genes results in a corresponding modification in the use of the frameshift versus error-free translesion pathways, suggesting a direct competition between Pol II and V for the bypass of the same lesion. Whether such a polymerase competition model for translesion synthesis will prove to be generally applicable remains to be confirmed. |
| Links |
PubMed PMC37476 Online version:10.1073/pnas.141113398 |
| Keywords |
2-Acetylaminofluorene/pharmacology; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; DNA Adducts; DNA Polymerase II/genetics; DNA Polymerase II/metabolism; DNA Replication; Deoxyribonucleases, Type II Site-Specific/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Frameshift Mutation; Gene Expression; Plasmids; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.