PMID:11443103

Citation	Hoffer, SM, Schoondermark, P, van Veen, HW and Tommassen, J (2001) Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12. J. Bacteriol. 183:4659-63
Abstract	Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene amplification event.
Links	PubMed PMC95363 Online version:10.1128/JB.183.15.4659-4663.2001
Keywords	ATP-Binding Cassette Transporters/genetics; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/metabolism; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Amplification; Genes, Bacterial; Molecular Sequence Data; Mutagenesis; Periplasmic Binding Proteins; Phosphate-Binding Proteins; Phosphates/metabolism
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