PMID:11440837

Citation	Oktyabrsky, ON, Smirnovam, GV and Muzyka, NG (2001) Role of glutathione in regulation of hydroperoxidase I in growing Escherichia coli. Free Radic. Biol. Med. 31:250-5
Abstract	To examine role of glutathione in regulation of catalases in growing Escherichia coli, katG::lacZ and katE::lacZ fusions were transformed into a glutathione-deficient Escherichia coli strain and wild-type parent. In the absence of H2O2 and in the presence of the low H2O2 concentrations (0.1-3 mM), the gshA mutation stimulated katG::lacZ expression and the total catalase activity in exponential phase. In the absence of H2O2, the mutation in gshA also stimulated katE::lacZ expression. At higher H2O2 concentrations, the gshA mutation suppressed katG::lacZ expression and catalase activity. In stationary and mid-exponential phases, the intracellular concentrations of H2O2 in the gshA mutant were markedly increased compared to those in the wild type. These results suggest that glutathione may be involved in regulation of catalases.
Links	PubMed
Keywords	Catalase/genetics; Catalase/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Genes, Bacterial; Glutathione/metabolism; Hydrogen Peroxide/metabolism; Lac Operon; Mutation; Oxidative Stress; Transformation, Genetic; beta-Galactosidase/genetics; beta-Galactosidase/metabolism
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