PMID:11440147

Citation	Matsui, H, Shimaoka, M, Takenaka, Y, Kawasaki, H and Kurahashi, O (2001) gsk disruption leads to guanosine accumulation in Escherichia coli. Biosci. Biotechnol. Biochem. 65:1230-5
Abstract	We tried some improvement of inosine production using an inosine-producing mutant of Escherichia coli which is deficient in purF (phosphoribosylpyrophosphate (PRPP) amidotransferase gene), purA (succinyl-adenosine 5'-monophosphate (AMP) synthetase gene), deoD (purine nucleoside phosphorylase gene), purR (purine repressor gene) and add (adenosine deaminase gene), and harboring the desensitized PRPP amidotransferase gene as a plasmid. The guaB (inosine 5'-monophosphate (IMP) dehydrogenase gene) disruption brought about a slightly positive effect on the inosine productivity. Alternatively, the gsk (guanosine-inosine kinase gene) disruption caused a considerable amount of guanosine accumulation together with a slight increase in the inosine productivity. The further addition of guaC (guanosine 5'-monophosphate (GMP) reductase gene) disruption did not lead to an increased guanosine accumulation, but brought about the decrease of inosine accumulation.
Links	PubMed
Keywords	Chromatography, High Pressure Liquid; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Guanosine/metabolism; Mutation; Phosphotransferases (Alcohol Group Acceptor)/genetics; Plasmids
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