PMID:11427897
| Citation |
Richard, SB, Bowman, ME, Kwiatkowski, W, Kang, I, Chow, C, Lillo, AM, Cane, DE and Noel, JP (2001) Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis. Nat. Struct. Biol. 8:641-8 |
|---|---|
| Abstract |
The YgbP protein of Escherichia coli encodes the enzyme 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthetase, a member of the cytidyltransferase family of enzymes. CDP-ME is an intermediate in the mevalonate-independent pathway for isoprenoid biosynthesis in a number of prokaryotic organisms, algae, the plant plastids and the malaria parasite. Because vertebrates synthesize isoprenoid precursors using a mevalonate pathway, CDP-ME synthetase and other enzymes of the mevalonate-independent pathway for isoprenoid production represent attractive targets for the structure-based design of selective antibacterial, herbicidal and antimalarial drugs. The high-resolution structures of E. coli CDP-ME synthetase in the apo form and complexed with both CTP-Mg2+ and CDP-ME-Mg2+ reveal the stereochemical principles underlying both substrate and product recognition as well as catalysis in CDP-ME synthetase. Moreover, these complexes represent the first experimental structures for any cytidyltransferase with both substrates and products bound. |
| Links |
PubMed Online version:10.1038/89691 |
| Keywords |
Apoenzymes/antagonists & inhibitors; Apoenzymes/chemistry; Apoenzymes/genetics; Apoenzymes/metabolism; Binding Sites; Catalysis; Crystallography, X-Ray; Cytidine Diphosphate/analogs & derivatives; Cytidine Diphosphate/chemistry; Cytidine Diphosphate/metabolism; Cytidine Triphosphate/chemistry; Cytidine Triphosphate/metabolism; Dimerization; Drug Design; Erythritol/analogs & derivatives; Erythritol/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Mevalonic Acid/metabolism; Models, Molecular; Mutation/genetics; Nucleotidyltransferases/antagonists & inhibitors; Nucleotidyltransferases/chemistry; Nucleotidyltransferases/genetics; Nucleotidyltransferases/metabolism; Polyisoprenyl Phosphates/biosynthesis; Polyisoprenyl Phosphates/chemistry; Polyisoprenyl Phosphates/metabolism; Protein Structure, Secondary; Structure-Activity Relationship; Substrate Specificity; Sugar Phosphates/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.