PMID:11427534
| Citation |
Han, YW, Iwasaki, H, Miyata, T, Mayanagi, K, Yamada, K, Morikawa, K and Shinagawa, H (2001) A unique beta-hairpin protruding from AAA+ ATPase domain of RuvB motor protein is involved in the interaction with RuvA DNA recognition protein for branch migration of Holliday junctions. J. Biol. Chem. 276:35024-8 |
|---|---|
| Abstract |
The Escherichia coli RuvB protein is a motor protein that forms a complex with RuvA and promotes branch migration of Holliday junctions during homologous recombination. This study describes the characteristics of two RuvB mutants, I148T and I150T, that do not promote branch migration in the presence of RuvA. These RuvB mutants hydrolyzed ATP and bound duplex DNA with the same efficiency as wild-type RuvB, but the mutants did not form a complex with RuvA and were defective in loading onto junction DNA in a RuvA-assisted manner. A recent crystallographic study revealed that Ile(148) and Ile(150) are in a unique beta-hairpin that protrudes from the AAA(+) ATPase domain of RuvB. We propose that this beta-hairpin interacts with hydrophobic residues in the mobile third domain of RuvA and that this interaction is vital for the RuvA-assisted loading of RuvB onto Holliday junction DNA. |
| Links |
PubMed Online version:10.1074/jbc.M103611200 |
| Keywords |
Adenosine Triphosphatases/chemistry; Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Proteins/chemistry; DNA/chemistry; DNA/metabolism; DNA Helicases; DNA Repair; DNA-Binding Proteins/chemistry; Escherichia coli Proteins; Molecular Sequence Data |
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