PMID:11415986

Citation	Urbanus, ML, Scotti, PA, Froderberg, L, Saaf, A, de Gier, JW, Brunner, J, Samuelson, JC, Dalbey, RE, Oudega, B and Luirink, J (2001) Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC. EMBO Rep. 2:524-9
Abstract	Recent studies identified YidC as a novel membrane factor that may play a key role in membrane insertion of inner membrane proteins (IMPs), both in conjunction with the Sec-translocase and as a separate entity. Here, we show that the type II IMP FtsQ requires both the translocase and, to a lesser extent, YidC in vivo. Using photo-crosslinking we demonstrate that the transmembrane (TM) domain of the nascent IMP FtsQ inserts into the membrane close to SecY and lipids, and moves to a combined YidC/lipid environment upon elongation. These data are consistent with a crucial role for YidC in the lateral transfer of TM domains from the Sec translocase into the lipid bilayer.
Links	PubMed PMC1083902 Online version:10.1093/embo-reports/kve108
Keywords	Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Carrier Proteins/metabolism; Cell Membrane/enzymology; Cell Membrane/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins; Lipid Metabolism; Membrane Proteins/metabolism; Membrane Transport Proteins; Models, Biological; Plasmids/metabolism; Protein Binding; Protein Structure, Tertiary; RNA, Messenger/metabolism; Ribosomes/metabolism
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