PMID:11415985

Citation	van der Laan, M, Houben, EN, Nouwen, N, Luirink, J and Driessen, AJ (2001) Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner. EMBO Rep. 2:519-23
Abstract	The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG-mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC-dependent membrane protein insertion via the SecYEG complex.
Links	PubMed PMC1083900 Online version:10.1093/embo-reports/kve106
Keywords	Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Carrier Proteins/metabolism; Cell Membrane/enzymology; Cell Membrane/metabolism; Codon; Cross-Linking Reagents/pharmacology; Dimerization; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Proteins/metabolism; Membrane Transport Proteins; Plasmids/metabolism; Protein Binding; Protein Biosynthesis; Proteolipids/metabolism; RNA, Messenger/metabolism; Transcription, Genetic; Translocation, Genetic
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