PMID:11394965
| Citation |
Lu, C, Stricker, J and Erickson, HP (2001) Site-specific mutations of FtsZ--effects on GTPase and in vitro assembly. BMC Microbiol. 1:7 |
|---|---|
| Abstract |
FtsZ, the major cytoskeletal protein in bacterial cytokinesis, assembles in vitro into protofilaments, which can further associate into sheets, bundles or tubes. We have constructed 16 site-directed mutants of E. coli ftsZ, and tested them for GTP hydrolysis and assembly in vitro, and for their ability to complement the temperature sensitive ftsZ84 mutation in E. coli. |
| Links | |
| Keywords |
Cell Division/physiology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; GTP Phosphohydrolases/metabolism; Genetic Complementation Test; Guanosine Triphosphate/metabolism; Mutagenesis, Site-Directed |
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