PMID:11375931

Citation	Agafonov, DE, Kolb, VA and Spirin, AS (2001) Ribosome-associated protein that inhibits translation at the aminoacyl-tRNA binding stage. EMBO Rep. 2:399-402
Abstract	We have recently isolated and characterized a novel protein associated with Escherichia coli ribosomes and named protein Y (pY). Here we show that the ribosomes from bacterial cells growing at a normal physiological temperature contain no pY, whereas a temperature downshift results in the appearance of the protein in ribosomes. The protein also appears in the ribosomes of those cells that reached the stationary phase of growth at a physiological temperature. Our experiments with cell-free translation systems demonstrate that the protein inhibits translation at the elongation stage by blocking the binding of aminoacyl-tRNA to the ribosomal A site. The function of the protein in adaptation of cells to environmental stress is discussed.
Links	PubMed PMC1083885 Online version:10.1093/embo-reports/kve091
Keywords	Bacterial Proteins/metabolism; Binding Sites; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Protein Binding; Protein Biosynthesis; RNA, Transfer, Amino Acyl/genetics; RNA, Transfer, Amino Acyl/metabolism; Ribosomal Proteins/metabolism; Ribosomes/genetics; Ribosomes/metabolism; Temperature
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