PMID:11371527
| Citation |
Bell, DC and Cupples, CG (2001) Very-short-patch repair in Escherichia coli requires the dam adenine methylase. J. Bacteriol. 183:3631-5 |
|---|---|
| Abstract |
Strains of Escherichia coli which lack the dam-encoded adenine methylase are mutators due to a reduction in the efficiency of postreplication mismatch repair. In this study, we show that Dam(-) strains are also defective in very-short-patch repair, the system which corrects T/G mismatches arising from the deamination of 5-methylcytosine. This defect is associated with decreased levels of Vsr, the endonuclease which initiates short-patch repair. We also show that production of the dcm-encoded cytosine methylase is unaffected in Dam(-) strains. Since the dcm and vsr genes are cotranscribed, the regulation of Vsr by Dam is probably posttranscriptional. |
| Links |
PubMed PMC95240 Online version:10.1128/JB.183.12.3631-3635.2001 |
| Keywords |
5-Methylcytosine; Adenosine Triphosphatases; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Pair Mismatch; Blotting, Western; Cytosine/analogs & derivatives; Cytosine/metabolism; DNA Methylation; DNA Repair; DNA Repair Enzymes; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Deamination; Endodeoxyribonucleases/genetics; Endodeoxyribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; Mutation; Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics; Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism |
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