PMID:11361334
| Citation |
Shanado, Y, Hanada, K and Ikeda, H (2001) Suppression of gamma ray-induced illegitimate recombination in Escherichia coli by the DNA-binding protein H-NS. Mol. Genet. Genomics 265:242-8 |
|---|---|
| Abstract |
To study the mechanism of gamma-ray-induced illegitimate recombination, we examined the formation of lambdabio transducing phage in Escherichia coli after gamma-ray irradiation. We show that gamma-ray irradiation enhances the formation of lambdabio transducing phage during prophage induction. Moreover, an hns mutation synergistically enhanced the incidence of lambda-ray-induced illegitimate recombination. Next we determined the sequences at the recombination junctions of the lambdabio transducing phages induced by gamma-ray irradiation. Most of the recombination sites coincided with known hotspots. Among them, hotspot I accounted for 67% and 77% of gamma-ray-induced lambdabio transducing phages in the wild type and the hns mutant, respectively. Therefore, the recombination sites appear to occur mostly at hotspot I or at other hotspots, but rarely at non-hotspot sites. These results suggest that types of DNA damage other than the double-strand breaks induced at random sites are mainly responsible for the introduction of the site-specific or region-specific DNA double strand breaks that lead to recombination at the hotspots. The results also showed that the recombination events took place between DNA sequences possessing short stretches of homology. H-NS protein, which binds to curved DNA, suppresses illegitimate recombination in the presence and absence of gamma-ray irradiation. Models for gamma-ray-induced illegitimate recombination are discussed. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/radiation effects; Gamma Rays; Mutagenesis; Recombination, Genetic; Ultraviolet Rays |
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