PMID:11353844
| Citation |
Weaver, LH, Kwon, K, Beckett, D and Matthews, BW (2001) Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator. Proc. Natl. Acad. Sci. U.S.A. 98:6045-50 |
|---|---|
| Abstract |
The Escherichia coli biotin repressor binds to the biotin operator to repress transcription of the biotin biosynthetic operon. In this work, a structure determined by x-ray crystallography of a complex of the repressor bound to biotin, which also functions as an activator of DNA binding by the biotin repressor (BirA), is described. In contrast to the monomeric aporepressor, the complex is dimeric with an interface composed in part of an extended beta-sheet. Model building, coupled with biochemical data, suggests that this is the dimeric form of BirA that binds DNA. Segments of three surface loops that are disordered in the aporepressor structure are located in the interface region of the dimer and exhibit greater order than was observed in the aporepressor structure. The results suggest that the corepressor of BirA causes a disorder-to-order transition that is a prerequisite to repressor dimerization and DNA binding. |
| Links |
PubMed PMC33419 Online version:10.1073/pnas.111128198 |
| Keywords |
Allosteric Regulation; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Base Sequence; Biotin/chemistry; Biotin/metabolism; Carbon-Nitrogen Ligases/chemistry; Carbon-Nitrogen Ligases/metabolism; Dimerization; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Operator Regions, Genetic; Protein Structure, Secondary; Repressor Proteins; Trans-Activators; Transcription Factors; Transcriptional Activation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.