PMID:11344143
| Citation |
Smulski, DR, Huang, LL, McCluskey, MP, Reeve, MJ, Vollmer, AC, Van Dyk, TK and LaRossa, RA (2001) Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12. J. Bacteriol. 183:3353-64 |
|---|---|
| Abstract |
Acivicin, a modified amino acid natural product, is a glutamine analog. Thus, it might interfere with metabolism by hindering glutamine transport, formation, or usage in processes such as transamidation and translation. This molecule prevented the growth of Escherichia coli in minimal medium unless the medium was supplemented with a purine or histidine, suggesting that the HisHF enzyme, a glutamine amidotransferase, was the target of acivicin action. This enzyme, purified from E. coli, was inhibited by low concentrations of acivicin. Acivicin inhibition was overcome by the presence of three distinct genetic regions when harbored on multicopy plasmids. Comprehensive transcript profiling using DNA microarrays indicated that histidine biosynthesis was the predominant process blocked by acivicin. The response to acivicin, however, was quite complex, suggesting that acivicin inhibition resonated through more than a single cellular process. |
| Links |
PubMed PMC99633 Online version:10.1128/JB.183.11.3353-3364.2001 |
| Keywords |
Aminohydrolases/genetics; Aminohydrolases/metabolism; Anthranilate Synthase; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding, Competitive; Culture Media; Enzyme Inhibitors/metabolism; Enzyme Inhibitors/pharmacology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Gene Expression Profiling/methods; Glutamine/metabolism; Imidazoles/metabolism; Isoxazoles/metabolism; Isoxazoles/pharmacology; Nitrogenous Group Transferases/antagonists & inhibitors; Nitrogenous Group Transferases/genetics; Nitrogenous Group Transferases/metabolism; Ribonucleotides/metabolism; Transaminases/genetics; Transaminases/metabolism |
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