PMID:11333217
| Citation |
Courcelle, J, Khodursky, A, Peter, B, Brown, PO and Hanawalt, PC (2001) Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158:41-64 |
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| Abstract |
The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by the LexA repressor. Using DNA microarrays containing amplified DNA fragments from 95.5% of all open reading frames identified on the E. coli chromosome, we have examined the changes in gene expression following UV exposure in both wild-type cells and lexA1 mutants, which are unable to induce genes under LexA control. We report here the time courses of expression of the genes surrounding the 26 documented lexA-regulated regions on the E. coli chromosome. We observed 17 additional sites that responded in a lexA-dependent manner and a large number of genes that were upregulated in a lexA-independent manner although upregulation in this manner was generally not more than twofold. In addition, several transcripts were either downregulated or degraded following UV irradiation. These newly identified UV-responsive genes are discussed with respect to their possible roles in cellular recovery following exposure to UV irradiation. |
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| Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli/radiation effects; Gene Expression Profiling; Oligonucleotide Array Sequence Analysis; SOS Response (Genetics); Serine Endopeptidases/genetics; Transcription, Genetic; Ultraviolet Rays |
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