PMID:11318649
| Citation |
Rothery, RA, Blasco, F and Weiner, JH (2001) Electron transfer from heme bL to the [3Fe-4S] cluster of Escherichia coli nitrate reductase A (NarGHI). Biochemistry 40:5260-8 |
|---|---|
| Abstract |
We have investigated the functional relationship between three of the prosthetic groups of Escherichia coli nitrate reductase A (NarGHI): the two hemes of the membrane anchor subunit (NarI) and the [3Fe-4S] cluster of the electron-transfer subunit (NarH). In two site-directed mutants (NarGHI(H56R) and NarGHI(H205Y)) that lack the highest potential heme of NarI (heme b(H)), a large negative DeltaE(m,7) is elicited on the NarH [3Fe-4S] cluster, suggesting a close juxtaposition of these two centers in the holoenzyme. In a mutant retaining heme b(H), but lacking heme b(L) (NarGHI(H66Y)), there is no effect on the NarH [3Fe-4S] cluster redox properties. These results suggest a role for heme b(H) in electron transfer to the [3Fe-4S] cluster. Studies of the pH dependence of the [3Fe-4S] cluster, heme b(H), and heme b(L) E(m) values suggest that significant deprotonation is only observed during oxidation of the latter heme (a pH dependence of -36 mV pH(-1)). In NarI expressed in the absence of NarGH [NarI(DeltaGH)], apparent exposure of heme b(H) to the aqueous milieu results in both it and heme b(L) having E(m) values with pH dependencies of approximately -30 mV pH(-1). These results are consistent with heme b(H) being isolated from the aqueous milieu and pH effects in the holoenzyme. Optical spectroscopy indicates that inhibitors such as HOQNO and stigmatellin bind and inhibit oxidation of heme b(L) but do not inhibit oxidation of heme b(H). Fluorescence quench titrations indicate that HOQNO binds with higher affinity to the reduced form of NarGHI than to the oxidized form. Overall, the data support the following model for electron transfer through the NarI region of NarGHI: Q(P) site --> heme b(L) --> heme b(H) --> [3Fe-4S] cluster. |
| Links | |
| Keywords |
Benzoquinones/chemistry; Dimerization; Electron Spin Resonance Spectroscopy; Electron Transport/genetics; Enzyme Inhibitors/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Heme/antagonists & inhibitors; Heme/chemistry; Heme/metabolism; Holoenzymes/genetics; Holoenzymes/metabolism; Hydrogen-Ion Concentration; Hydroquinones/chemistry; Hydroxyquinolines/chemistry; Iron-Sulfur Proteins/antagonists & inhibitors; Iron-Sulfur Proteins/genetics; Iron-Sulfur Proteins/metabolism; Multigene Family; Mutagenesis, Site-Directed; Nitrate Reductases/antagonists & inhibitors; Nitrate Reductases/genetics; Nitrate Reductases/metabolism; Oxidation-Reduction/drug effects; Polyenes/chemistry; Potentiometry; Protein Binding; Reducing Agents/chemistry; Spectrophotometry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.