PMID:11316870
| Citation |
Cronk, JD, Endrizzi, JA, Cronk, MR, O'neill, JW and Zhang, KY (2001) Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity. Protein Sci. 10:911-22 |
|---|---|
| Abstract |
Carbonic anhydrases fall into three distinct evolutionary and structural classes: alpha, beta, and gamma. The beta-class carbonic anhydrases (beta-CAs) are widely distributed among higher plants, simple eukaryotes, eubacteria, and archaea. We have determined the crystal structure of ECCA, a beta-CA from Escherichia coli, to a resolution of 2.0 A. In agreement with the structure of the beta-CA from the chloroplast of the red alga Porphyridium purpureum, the active-site zinc in ECCA is tetrahedrally coordinated by the side chains of four conserved residues. These results confirm the observation of a unique pattern of zinc ligation in at least some beta-CAS: The absence of a water molecule in the inner coordination sphere is inconsistent with known mechanisms of CA activity. ECCA activity is highly pH-dependent in the physiological range, and its expression in yeast complements an oxygen-sensitive phenotype displayed by a beta-CA-deletion strain. The structural and biochemical characterizations of ECCA presented here and the comparisons with other beta-CA structures suggest that ECCA can adopt two distinct conformations displaying widely divergent catalytic rates. |
| Links |
PubMed PMC2374203 Online version:10.1110/ps.46301 |
| Keywords |
Amino Acid Sequence; Binding Sites; Carbonic Anhydrases/chemistry; Carbonic Anhydrases/isolation & purification; Carbonic Anhydrases/metabolism; Crystallography, X-Ray; Escherichia coli/enzymology; Gene Deletion; Genetic Complementation Test; Hydrogen-Ion Concentration; Models, Molecular; Molecular Sequence Data; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Structure-Activity Relationship; Zinc/metabolism |
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