PMID:11309126
| Citation |
Takeda, S, Fujisawa, Y, Matsubara, M, Aiba, H and Mizuno, T (2001) A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour. Mol. Microbiol. 40:440-50 |
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| Abstract |
In this study, we re-investigated the previously characterized RcsC (sensor His-kinase) --> RcsB (response regulator) phosphorelay system that is involved in the regulation of capsular polysaccharide synthesis in Escherichia coli. The previously proposed model hypothesized the occurrence of a direct phosphotransfer from RcsC to RcsB in response to an unknown external stimulus. As judged from the current general view as to the His --> Asp phosphorelay, this RcsC --> RcsB framework is somewhat puzzling, because RcsC appears to contain both a His-kinase domain and a receiver domain, but not a histidine (His)-containing phosphotransmitter domain (e.g. HPt domain). We thus suspected that an as yet unknown mechanism might be underlying in this particular His --> Asp phosphorelay system. Here, we provide several lines of in vivo and in vitro evidence that a novel and unique His-containing phosphotransmitter (named YojN) is essential for this signalling system. A revised model is proposed in which the multistep RcsC --> YojN --> RcsB phosphorelay is implicated. It was also demonstrated that this complex signalling system is somehow involved in the modulation of a characteristic behaviour of E. coli cells during colony formation on the surface of agar plates, namely swarming. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Capsules/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Culture Media; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Multienzyme Complexes; Phosphoprotein Phosphatases; Phosphorylation; Phosphotransferases; Plasmids; Protein Kinases; Signal Transduction; Transcription Factors/genetics; Transcription Factors/metabolism |
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