PMID:11292818

Citation	Jishage, M, Dasgupta, D and Ishihama, A (2001) Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase. J. Bacteriol. 183:2952-6
Abstract	Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma(70) subunit. The contact site of Rsd on sigma(70) was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma(70) and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma(70) contact transcription factors.
Links	PubMed PMC99515 Online version:10.1128/JB.183.9.2952-2956.2001
Keywords	Alanine; Amino Acid Sequence; Amino Acid Substitution; Binding Sites; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Molecular Sequence Data; Mutation; Protein Binding; Repressor Proteins/genetics; Repressor Proteins/isolation & purification; Repressor Proteins/metabolism; Sensitivity and Specificity; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription, Genetic
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