PMID:11292795
| Citation |
Mishra, P, Park, PK and Drueckhammer, DG (2001) Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12. J. Bacteriol. 183:2774-8 |
|---|---|
| Abstract |
Dephosphocoenzyme A (dephospho-CoA) kinase catalyzes the final step in coenzyme A biosynthesis, the phosphorylation of the 3'-hydroxy group of the ribose sugar moiety. Wild-type dephospho-CoA kinase from Corynebacterium ammoniagenes was purified to homogeneity and subjected to N-terminal sequence analysis. A BLAST search identified a gene from Escherichia coli previously designated yacE encoding a highly homologous protein. Amplification of the gene and overexpression yielded recombinant dephospho-CoA kinase as a 22.6-kDa monomer. Enzyme assay and nuclear magnetic resonance analyses of the product demonstrated that the recombinant enzyme is indeed dephospho-CoA kinase. The activities with adenosine, AMP, and adenosine phosphosulfate were 4 to 8% of the activity with dephospho-CoA. Homologues of the E. coli dephospho-CoA kinase were identified in a diverse range of organisms. |
| Links |
PubMed PMC99492 Online version:10.1128/JB.183.9.2774-2778.2001 |
| Keywords |
Adenosine/metabolism; Adenosine Monophosphate/metabolism; Adenosine Phosphosulfate/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cloning, Molecular; Corynebacterium/enzymology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Bacterial; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor)/genetics; Phosphotransferases (Alcohol Group Acceptor)/isolation & purification; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Recombinant Proteins/genetics; Sequence Alignment |
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