PMID:11287152
| Citation |
Prüss, BM, Liu, X, Hendrickson, W and Matsumura, P (2001) FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions. FEMS Microbiol. Lett. 197:91-7 |
|---|---|
| Abstract |
The Escherichia coli transcriptional regulatory complex FlhD/FlhC, initially identified as a flagella-specific activator, is a global regulator involved in many cellular processes. Using gene arrays, lacZ gene fusions and enzyme assays, eight new targets of FlhD/FlhC were recognized. These are the transporter for galactose (MglBAC), the rod-shape determination proteins (MreBCD), malate dehydrogenase, and several enzymes involved in anaerobic respiration (glycerol 3-phosphate dehydrogenase, GlpABC; periplasmic nitrate reductase, NapFAGHBC; nitrite reductase, NrfABCDEFG; dimethyl sulfoxide reductase, DmsABC; and the modulator for hydrogenases, HydNHypF). |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Flagella/genetics; Flagella/metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Lac Operon/physiology; Malate Dehydrogenase/genetics; Malate Dehydrogenase/metabolism; Oligonucleotide Array Sequence Analysis/methods; Promoter Regions, Genetic/genetics; Promoter Regions, Genetic/physiology; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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