PMID:11287001
| Citation |
Mikhaleva, NI, Golovastov, VV, Zolov, SN, Bogdanov, MV, Dowhan, W and Nesmeyanova, MA (2001) Depletion of phosphatidylethanolamine affects secretion of Escherichia coli alkaline phosphatase and its transcriptional expression. FEBS Lett. 493:85-90 |
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| Abstract |
In this report we demonstrate that depletion of the major phospholipid phosphatidylethanolamine, a single non-bilayer forming phospholipid of Escherichia coli, significantly reduces the secretion efficiency of alkaline phosphatase in vivo. Secretion, however, is correlated with the content in membranes of cardiolipin, which in combination with selected divalent cations has a strong tendency to adopt a non-bilayer state indicating the possible involvement of lipid polymorphism in efficient protein secretion. Depletion of this zwitterionic phospholipid also inhibits expression of the protein controlled by the endogenous P(PHO) promoter but not the P(BAD) promoter, which is suggested to be due to the effect of unbalanced phospholipid composition on the orthophosphate signal transduction system (Pho regulon) through an effect on its membrane bound sensor. |
| Links | |
| Keywords |
Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Alkaline Phosphatase/secretion; Base Sequence; Biological Transport, Active; Cardiolipins/metabolism; Cell Membrane/metabolism; DNA Primers/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Enzymologic; Membrane Lipids/metabolism; Phosphatidylethanolamines/metabolism; Promoter Regions, Genetic; Transcription, Genetic |
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