PMID:11283292
| Citation |
Franke, S, Grass, G and Nies, DH (2001) The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions. Microbiology (Reading, Engl.) 147:965-72 |
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| Abstract |
Transcription of the ybcZ--ylcA ylcBCD--ybdE region of the Escherichia coli K38 chromosome was analysed by Northern RNA--DNA hybridization, RT-PCR and primer extension. Transcription of a dicistronic ybcZ--ylcA mRNA and a tetracistronic ylcBCD--ybdE mRNA was induced by silver and was initiated from the sigma-70 promoters ylcAp and ylcBp. Expression of beta-galactosidase activity from a Phi(ylcBp--lacZ) operon fusion was also induced by Ag(+) and Cu(2+), but not by Zn(2+). In-frame deletion of ybdE from the chromosome yielded a silver-sensitive E. coli mutant strain which did not differ in its copper resistance from its wild-type strain. On the other hand, deletion of the copA gene for the copper-exporting P-type ATPase CopA resulted in copper sensitivity, but not in silver sensitivity. A Delta ybdE Delta copA double mutant strain behaved towards copper as the Delta copA strain and towards silver as the Delta ybdE strain. Thus, in E. coli, the YlcBCD--YbdE system may be involved in silver- but not in copper resistance, and CopA may be involved in copper- but not in silver resistance. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Northern; Chromosomes, Bacterial; Copper/metabolism; Copper/toxicity; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Genes, Reporter; Lac Operon; Operon; Promoter Regions, Genetic; RNA, Bacterial/analysis; Reverse Transcriptase Polymerase Chain Reaction; Sequence Deletion; Silver/metabolism; Silver/toxicity |
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