PMID:11274135

Citation	Galvani, C, Terry, J and Ishiguro, EE (2001) Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes. J. Bacteriol. 183:2700-3
Abstract	The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.
Links	PubMed PMC95192 Online version:10.1128/JB.183.8.2700-2703.2001
Keywords	Antitoxins/genetics; Antitoxins/isolation & purification; Antitoxins/metabolism; Bacterial Toxins/genetics; Bacterial Toxins/isolation & purification; Bacterial Toxins/metabolism; Bacterial Toxins/toxicity; Cytotoxins/genetics; Cytotoxins/isolation & purification; Cytotoxins/metabolism; Cytotoxins/toxicity; Escherichia coli/genetics; Escherichia coli/metabolism; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/isolation & purification; Proto-Oncogene Proteins/metabolism; Ribosomes/metabolism; Transcription Factor RelB; Transcription Factors/genetics; Transcription Factors/isolation & purification; Transcription Factors/metabolism; Two-Hybrid System Techniques
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