PMID:11269500
| Citation |
van Dalen, A, Schrempf, H, Killian, JA and de Kruijff, B (2000) Efficient membrane assembly of the KcsA potassium channel in Escherichia coli requires the protonmotive force. EMBO Rep. 1:340-6 |
|---|---|
| Abstract |
Very little is known about the biogenesis and assembly of oligomeric membrane proteins. In this study, the biogenesis of KcsA, a prokaryotic homotetrameric potassium channel, is investigated. Using in vivo pulse-chase experiments, both the monomeric and tetrameric form could be identified. The conversion of monomers into a tetramer is found to be a highly efficient process that occurs in the Escherichia coli inner membrane. KcsA does not require ATP hydrolysis by SecA for insertion or tetramerization. The presence of the proton-motive force (pmf) is not necessary for transmembrane insertion of KcsA; however, the pmf proved to be essential for the efficiency of oligomerization. From in vivo and in vitro experiments it is concluded that the electrical component, deltapsi, is the main determinant for this effect. These results demonstrate a new role of the pmf in membrane protein biogenesis. |
| Links |
PubMed PMC1083744 Online version:10.1093/embo-reports/kvd067 |
| Keywords |
Adenosine Triphosphate/metabolism; Azides/pharmacology; Bacterial Outer Membrane Proteins/analysis; Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/physiology; Biogenesis; Biological Transport; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Cell Membrane/physiology; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors/pharmacology; Escherichia coli/physiology; Potassium Channel Blockers; Potassium Channels/physiology; Protein Biosynthesis; Proton-Motive Force/physiology; Transcription, Genetic; Trypsin/pharmacology; Uncoupling Agents/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.