PMID:11266368
| Citation |
Py, B, Loiseau, L and Barras, F (2001) An inner membrane platform in the type II secretion machinery of Gram-negative bacteria. EMBO Rep. 2:244-8 |
|---|---|
| Abstract |
The type II secretion machinery allows most Gram-negative bacteria to deliver virulence factors into their surroundings. We report that in Erwinia chrysanthemi, GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assembling other parts of the secretion machinery. The GspE-GspF-GspL-GspM complex was demonstrated by two methods: (i) co-immunoprecipitation of GspE-GspF-GspL with antibodies raised against either GspE or GspF; (ii) interactions in the yeast two-hybrid system between GspF and GspE, GspF and GspL, GspL and GspM. GspL was found to have an essential role in complex formation. We propose a model in which the GspE-GspF-GspL-GspM proteins constitute a building block within the secretion machinery on top of which another building block, referred to as a pseudopilus, assembles. By analogy, we predict that a similar platform is required for the biogenesis of the type IV pilus. |
| Links |
PubMed PMC1083838 Online version:10.1093/embo-reports/kve042 |
| Keywords |
Bacterial Outer Membrane Proteins/metabolism; Carrier Proteins/metabolism; Cell Membrane/metabolism; Gram-Negative Bacteria/genetics; Gram-Negative Bacteria/metabolism; Gram-Negative Bacteria/pathogenicity; Models, Biological; Pectobacterium chrysanthemi/genetics; Pectobacterium chrysanthemi/metabolism; Pectobacterium chrysanthemi/pathogenicity; Two-Hybrid System Techniques; Virulence |
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