PMID:11263493
| Citation |
Weitao, T, Nordström, K and Dasgupta, S (2000) Escherichia coli cell cycle control genes affect chromosome superhelicity. EMBO Rep. 1:494-9 |
|---|---|
| Abstract |
We have used ethidium bromide titration for direct measurement of the changes in the negative supercoiling of Escherichia coli chromosome caused by mutations inactivating the cell cycle functions mukB and seqA. The amounts of the intercalative agent required to relax the supercoiled chromosome in mukB and seqA mutants were lower and higher, respectively, than for the wild-type parent, confirming that these cell cycle genes modulate the topology of the E. coli chromosome. Plasmid superhelicity measured in these mutant strains showed similar effects albeit of reduced magnitude. As the effects of mukB and seqA mutations were not restricted to the chromosome alone, MukB and SeqA proteins possibly interact with factors involved in the maintenance of intracellular DNA topology. To our knowledge, this is the first direct demonstration of the influence of mukB and seqA genes on the superhelicity of the E. coli chromosome. |
| Links |
PubMed PMC1083778 Online version:10.1093/embo-reports/kvd106 |
| Keywords |
Bacterial Outer Membrane Proteins; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Cell Cycle/genetics; Cell Membrane/metabolism; Centrifugation, Density Gradient; Chloroquine/pharmacology; Chromosomal Proteins, Non-Histone; Chromosomes/ultrastructure; DNA, Superhelical/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Deoxyribonuclease EcoRI/metabolism; Dose-Response Relationship, Drug; Electrophoresis, Agar Gel; Escherichia coli/genetics; Escherichia coli Proteins; Ethidium/pharmacology; Intercalating Agents/pharmacology; Mutation; Plasmids/metabolism; Sucrose/pharmacology; Transcription Factors |
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