PMID:11258701
| Citation |
Lin, IG and Hsieh, CL (2001) Chromosomal DNA demethylation specified by protein binding. EMBO Rep. 2:108-12 |
|---|---|
| Abstract |
In the present study, we utilize the well-characterized Escherichia coli lac repressor/operator system to demonstrate that protein binding can lead to demethylation at the binding sites in the chromosome. Similar to the findings using the episome, we found that the presence of LacI in the cells can lead to demethylation of methylated lacO in the chromosome and the LacI inhibitor, isopropyl-beta-D-thiogalactopyranoside (IPTG), can prevent demethylation of the methylated lacO. The lacO sites become progressively more demethylated over time with the presence of LacI, supporting the role of protein occupancy in demethylation targeting. These results validate our earlier conclusions using a stable episomal system, and establish for the first time that protein binding can specify sites of demethylation in the chromosome. |
| Links |
PubMed PMC1083819 Online version:10.1093/embo-reports/kve023 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Southern; Cell Line; Chromosomes, Human/genetics; Chromosomes, Human/metabolism; DNA Methylation/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Genome, Human; Humans; Isopropyl Thiogalactoside/pharmacology; Lac Operon/genetics; Lac Repressors; Protein Binding/drug effects; Recombination, Genetic/genetics; Repressor Proteins/genetics; Repressor Proteins/metabolism; Time Factors; Transfection |
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