PMID:11254985
| Citation |
Pas, E, Einav, M, Woldringh, CL and Zaritsky, A (2001) Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells. Biochimie 83:121-4 |
|---|---|
| Abstract |
Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them. |
| Links | |
| Keywords |
Amdinocillin/pharmacology; Bacterial Proteins/metabolism; Cell Division; Cytoskeletal Proteins; Escherichia coli/cytology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Fluorescent Dyes/metabolism; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Microscopy, Fluorescence; Recombinant Fusion Proteins/metabolism |
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