PMID:11243816
| Citation |
Batey, RT, Sagar, MB and Doudna, JA (2001) Structural and energetic analysis of RNA recognition by a universally conserved protein from the signal recognition particle. J. Mol. Biol. 307:229-46 |
|---|---|
| Abstract |
The signal recognition particle (SRP) is a ribonucleoprotein complex responsible for targeting proteins to the endoplasmic reticulum in eukarya or to the inner membrane in prokarya. The crystal structure of the universally conserved RNA-protein core of the Escherichia coli SRP, refined here to 1.5 A resolution, revealed minor groove recognition of the 4.5 S RNA component by the M domain of the Ffh protein. Within the RNA, nucleotides comprising two phylogenetically conserved internal loops create a unique surface for protein recognition. To determine the energetic importance of conserved nucleotides for SRP assembly, we measured the affinity of the M domain for a series of RNA mutants. This analysis reveals how conserved nucleotides within the two internal loop motifs establish the architecture of the macromolecular interface and position essential functional groups for direct recognition by the protein. |
| Links |
PubMed Online version:10.1006/jmbi.2000.4454 |
| Keywords |
Conserved Sequence; Crystallization; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/metabolism; Humans; Models, Molecular; Nucleic Acid Conformation; Protein Conformation; Protein Structure, Tertiary; RNA, Bacterial; RNA, Ribosomal/chemistry; RNA, Ribosomal/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/metabolism; Signal Recognition Particle/chemistry; Signal Recognition Particle/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.