PMID:11160893
| Citation |
O'Connor, M, Lee, WM, Mankad, A, Squires, CL and Dahlberg, AE (2001) Mutagenesis of the peptidyltransferase center of 23S rRNA: the invariant U2449 is dispensable. Nucleic Acids Res. 29:710-5 |
|---|---|
| Abstract |
U2449 is one of many invariant residues in the central loop of domain V of 23S rRNA, a region that constitutes part of the peptidyltransferase center of the ribosome. In Escherichia coli, this U is post-transcriptionally modified to dihydrouridine (D) and is the only D modification found in E.coli rRNAs. To analyze the role of this base and its modification in ribosomal function, all three base substitutions were constructed on a plasmid copy of the rrnB operon and assayed for their ability to support cell growth in a strain of E.coli lacking chromosomal rrn operons. Both purine substitution mutations were not viable. However, growth and antibiotic sensitivity of cells expressing only the mutant D2449C rRNA was indistinguishable from wild type. We conclude that while a pyrimidine is required at position 2449 for proper ribosomal function, the D modification is dispensable. |
| Links | |
| Keywords |
Base Sequence; DNA, Recombinant; Escherichia coli/genetics; Escherichia coli/growth & development; Molecular Sequence Data; Mutagenesis; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Peptidyl Transferases/metabolism; Plasmids/genetics; RNA Processing, Post-Transcriptional; RNA, Ribosomal, 23S/chemistry; RNA, Ribosomal, 23S/genetics; RNA, Ribosomal, 23S/metabolism; Uridine/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.